Journal: International Journal of Molecular Sciences
Article Title: Modulation of the Arabidopsis Starch Metabolic Network by the Cytosolic Acetyl-CoA Pathway in the Context of the Diurnal Illumination Cycle
doi: 10.3390/ijms251910850
Figure Lengend Snippet: Expression profiles of genes encoding enzymes related to starch metabolism during the SD diurnal cycle. Genes are grouped by their biochemical activity: ( A ) starch synthases (SSs), ( B ) starch branching enzymes (BEs), ( C ) starch debranching enzymes (DBEs), ( D ) starch disproportionating enzymes (DPEs), ( E ) β-amylases (BAMs), ( F ) α-amylases (AAMs), ( G ) starch phosphorylases (PHSs), and ( H ) other enzymes related to starch metabolism. Leaves from WT plants cultivated under short day (SD) conditions, consisting of 8 h of light followed by 16 h of dark, were collected at various times throughout the 24-h cycle. RNA from these samples was then examined using the Affymetrix Arabidopsis ATH1 chip. The average normalized intensity for each microarray chip is 1. RNA was analyzed using GeneChip. The analysis was repeated in two independent experiments.
Article Snippet: Synthesis of labeled cRNAs, hybridization with Affymetrix Arabidopsis ATH1 arrays (22,810 Arabidopsis probe sets, Affymetrix, Santa Clara, CA, USA), and scanning of the probe arrays were performed at the Iowa State University Gene Chip Facility (Ames, IA, USA).
Techniques: Expressing, Starch, Activity Assay, Microarray