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ath1 genome array  (Thermo Fisher)


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    Thermo Fisher ath1 genome array
    Ath1 Genome Array, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 1 article reviews
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    Expression of hypoxia-responsive genes during Botrytis infection A) bar plot of log 2 fold change (log 2 FC) expression values for core anaerobic genes after 48 h from the inoculation with Botrytis. Genes are sorted in descending order, with a dashed line indicating the threshold of log 2 FC = 1, representing significantly upregulated genes in adult <t>Arabidopsis</t> plants rosette 48 h post infection. B) Expression analysis of Group B core anaerobic genes after 48 h from infection with Botrytis and upon low-oxygen stresses. The treatments were 4 h of gaseous hypoxia and 4 h of submergence. Data ( n = 5) are relative to the mock group for Botrytis and to the air group for hypoxia/submergence treatments, both set to 1. C) Expression analysis of Group A core anaerobic genes after 48 h from infection with Botrytis and upon low-oxygen stress. The treatments were as in panel B) . Statistically significant differences in B) and C) are indicated by the asterisks (Student t -test, unpaired comparison was used for mock to Botrytis comparison, one-way ANOVA followed by Dunnett's multiple comparisons test was used for air to hypoxia and submergence comparisons. * = P < 0.05; ** = P < 0.01; *** = P < 0.001; **** = P < 0.0001). Lines within the boxes indicate the median, while the bottom and top of each box denote the first and third quartile, respectively, the dots represent the single data points and whiskers denote the min/max values.
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    Expression profiles of genes encoding enzymes related to starch metabolism during the SD diurnal cycle. Genes are grouped by their biochemical activity: ( A ) starch synthases (SSs), ( B ) starch branching enzymes (BEs), ( C ) starch debranching enzymes (DBEs), ( D ) starch disproportionating enzymes (DPEs), ( E ) β-amylases (BAMs), ( F ) α-amylases (AAMs), ( G ) starch phosphorylases (PHSs), and ( H ) other enzymes related to starch metabolism. Leaves from WT plants cultivated under short day (SD) conditions, consisting of 8 h of light followed by 16 h of dark, were collected at various times throughout the 24-h cycle. RNA from these samples was then examined using the Affymetrix <t>Arabidopsis</t> <t>ATH1</t> chip. The average normalized intensity for each microarray chip is 1. RNA was analyzed using GeneChip. The analysis was repeated in two independent experiments.
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    Expression profiles of genes encoding enzymes related to starch metabolism during the SD diurnal cycle. Genes are grouped by their biochemical activity: ( A ) starch synthases (SSs), ( B ) starch branching enzymes (BEs), ( C ) starch debranching enzymes (DBEs), ( D ) starch disproportionating enzymes (DPEs), ( E ) β-amylases (BAMs), ( F ) α-amylases (AAMs), ( G ) starch phosphorylases (PHSs), and ( H ) other enzymes related to starch metabolism. Leaves from WT plants cultivated under short day (SD) conditions, consisting of 8 h of light followed by 16 h of dark, were collected at various times throughout the 24-h cycle. RNA from these samples was then examined using the Affymetrix <t>Arabidopsis</t> <t>ATH1</t> chip. The average normalized intensity for each microarray chip is 1. RNA was analyzed using GeneChip. The analysis was repeated in two independent experiments.
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    Thermo Fisher arabidopsis ath1 genome array data
    Expression profiles of genes encoding enzymes related to starch metabolism during the SD diurnal cycle. Genes are grouped by their biochemical activity: ( A ) starch synthases (SSs), ( B ) starch branching enzymes (BEs), ( C ) starch debranching enzymes (DBEs), ( D ) starch disproportionating enzymes (DPEs), ( E ) β-amylases (BAMs), ( F ) α-amylases (AAMs), ( G ) starch phosphorylases (PHSs), and ( H ) other enzymes related to starch metabolism. Leaves from WT plants cultivated under short day (SD) conditions, consisting of 8 h of light followed by 16 h of dark, were collected at various times throughout the 24-h cycle. RNA from these samples was then examined using the Affymetrix <t>Arabidopsis</t> <t>ATH1</t> chip. The average normalized intensity for each microarray chip is 1. RNA was analyzed using GeneChip. The analysis was repeated in two independent experiments.
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    Expression of hypoxia-responsive genes during Botrytis infection A) bar plot of log 2 fold change (log 2 FC) expression values for core anaerobic genes after 48 h from the inoculation with Botrytis. Genes are sorted in descending order, with a dashed line indicating the threshold of log 2 FC = 1, representing significantly upregulated genes in adult Arabidopsis plants rosette 48 h post infection. B) Expression analysis of Group B core anaerobic genes after 48 h from infection with Botrytis and upon low-oxygen stresses. The treatments were 4 h of gaseous hypoxia and 4 h of submergence. Data ( n = 5) are relative to the mock group for Botrytis and to the air group for hypoxia/submergence treatments, both set to 1. C) Expression analysis of Group A core anaerobic genes after 48 h from infection with Botrytis and upon low-oxygen stress. The treatments were as in panel B) . Statistically significant differences in B) and C) are indicated by the asterisks (Student t -test, unpaired comparison was used for mock to Botrytis comparison, one-way ANOVA followed by Dunnett's multiple comparisons test was used for air to hypoxia and submergence comparisons. * = P < 0.05; ** = P < 0.01; *** = P < 0.001; **** = P < 0.0001). Lines within the boxes indicate the median, while the bottom and top of each box denote the first and third quartile, respectively, the dots represent the single data points and whiskers denote the min/max values.

    Journal: Plant Physiology

    Article Title: The transcription factor ORA59 represses hypoxia responses during Botrytis cinerea infection and reoxygenation

    doi: 10.1093/plphys/kiae677

    Figure Lengend Snippet: Expression of hypoxia-responsive genes during Botrytis infection A) bar plot of log 2 fold change (log 2 FC) expression values for core anaerobic genes after 48 h from the inoculation with Botrytis. Genes are sorted in descending order, with a dashed line indicating the threshold of log 2 FC = 1, representing significantly upregulated genes in adult Arabidopsis plants rosette 48 h post infection. B) Expression analysis of Group B core anaerobic genes after 48 h from infection with Botrytis and upon low-oxygen stresses. The treatments were 4 h of gaseous hypoxia and 4 h of submergence. Data ( n = 5) are relative to the mock group for Botrytis and to the air group for hypoxia/submergence treatments, both set to 1. C) Expression analysis of Group A core anaerobic genes after 48 h from infection with Botrytis and upon low-oxygen stress. The treatments were as in panel B) . Statistically significant differences in B) and C) are indicated by the asterisks (Student t -test, unpaired comparison was used for mock to Botrytis comparison, one-way ANOVA followed by Dunnett's multiple comparisons test was used for air to hypoxia and submergence comparisons. * = P < 0.05; ** = P < 0.01; *** = P < 0.001; **** = P < 0.0001). Lines within the boxes indicate the median, while the bottom and top of each box denote the first and third quartile, respectively, the dots represent the single data points and whiskers denote the min/max values.

    Article Snippet: Affymetrix GeneChip Arabidopsis ATH1 Genome Arrays were used to process RNA, as previously described ( ).

    Techniques: Expressing, Infection, Comparison

    Effects of jasmonate treatments on the expression of HRGs . A) Time-course expression analysis of jasmonate-responsive marker genes. Statistically significant differences are indicated by asterisks after Kruskal–Wallis test followed by Dunn's multiple comparisons test (* = P < 0.05; ** = P < 0.01; *** = P < 0.001; **** = P < 0.0001). Data ( n = 6) are relative to time 0 group set to 1. B) Expression analysis of Group B and A core anaerobic genes in adult Arabidopsis plants rosette pretreated with 50 µM MeJA for 7 h and then submerged in the dark for 2 h. Different letters indicate significant difference ( P < 0.05) after two-ways ANOVA followed by Tukey's post-hoc test. Data ( n = 6) are relative to the Col-0 control (air) not pretreated with 50 μM MeJA, set to 1. C) Representative images of LUC activity in pADH:LUC and 5xHRPE:LUC plants after 7 h incubation with 50 µM MeJA and 2 h of gaseous hypoxia. The scale bar is 1 cm. D) Quantification of LUC activity (counts per second, CPS) of pADH:LUC and 5xHRPE:LUC reporter lines showed in C) ( n ≥ 5). Quantification of CPS was carried out using the IndiGO software, by selecting a fixed area around the plants. Statistically significant differences are indicated by the asterisks (Student t -test, unpaired comparison; * = P < 0.05; ** = P < 0.01; *** = P < 0.001; **** = P < 0.0001). Lines within the boxes indicate the median, while the bottom and top of each box denote the first and third quartile, respectively, the dots represent the single data points and whiskers denote the min/max values.

    Journal: Plant Physiology

    Article Title: The transcription factor ORA59 represses hypoxia responses during Botrytis cinerea infection and reoxygenation

    doi: 10.1093/plphys/kiae677

    Figure Lengend Snippet: Effects of jasmonate treatments on the expression of HRGs . A) Time-course expression analysis of jasmonate-responsive marker genes. Statistically significant differences are indicated by asterisks after Kruskal–Wallis test followed by Dunn's multiple comparisons test (* = P < 0.05; ** = P < 0.01; *** = P < 0.001; **** = P < 0.0001). Data ( n = 6) are relative to time 0 group set to 1. B) Expression analysis of Group B and A core anaerobic genes in adult Arabidopsis plants rosette pretreated with 50 µM MeJA for 7 h and then submerged in the dark for 2 h. Different letters indicate significant difference ( P < 0.05) after two-ways ANOVA followed by Tukey's post-hoc test. Data ( n = 6) are relative to the Col-0 control (air) not pretreated with 50 μM MeJA, set to 1. C) Representative images of LUC activity in pADH:LUC and 5xHRPE:LUC plants after 7 h incubation with 50 µM MeJA and 2 h of gaseous hypoxia. The scale bar is 1 cm. D) Quantification of LUC activity (counts per second, CPS) of pADH:LUC and 5xHRPE:LUC reporter lines showed in C) ( n ≥ 5). Quantification of CPS was carried out using the IndiGO software, by selecting a fixed area around the plants. Statistically significant differences are indicated by the asterisks (Student t -test, unpaired comparison; * = P < 0.05; ** = P < 0.01; *** = P < 0.001; **** = P < 0.0001). Lines within the boxes indicate the median, while the bottom and top of each box denote the first and third quartile, respectively, the dots represent the single data points and whiskers denote the min/max values.

    Article Snippet: Affymetrix GeneChip Arabidopsis ATH1 Genome Arrays were used to process RNA, as previously described ( ).

    Techniques: Expressing, Marker, Control, Activity Assay, Incubation, Software, Comparison

    ORA59 interacts with the ERFVIIs. A) Western blot analysis of RAP2.3 3xHA abundance in adult Arabidopsis plants leaves 48 h post infection with Botrytis followed by the quantification of the signal carried out using ImageLab TM software (BioRad). 40 μg of protein was loaded for each sample. Four biological replicates are shown. B) Expression analysis of ORA59 in Arabidopsis rosettes 48 h post infection. Data ( n = 6) are relative to the mock group set to 1. Statistically significant differences are indicated by the asterisks (Student t -test, unpaired comparison; * = P < 0.05; ** = P < 0.01; *** = P < 0.001; **** = P < 0.0001). C) Yeast two-hybrid assay between the 5 ERFVIIs and ORA59 (N-terminal 60 amino acids truncated, ORA59Δ1–60). D) As in C) , followed by a β-galactosidase assay (CPRG) test ( n = 4). E) Split-LUC assay of ORA59 fused to the N-terminal part of the firefly LUC and the ERFVIIs members (and negative control, HOL2) fused to the C-terminal part of the firefly LUC each ( n = 6). For D) and E) , statistically significant differences are indicated by asterisks after a one-way ANOVA followed by Dunnett's multiple comparisons test (* = P < 0.05; ** = P < 0.01; *** = P < 0.001; **** = P < 0.0001). Lines within the boxes indicate the median, while the bottom and top of each box denote the first and third quartile, respectively, the dots represent the single data points and whiskers denote the min/max values.

    Journal: Plant Physiology

    Article Title: The transcription factor ORA59 represses hypoxia responses during Botrytis cinerea infection and reoxygenation

    doi: 10.1093/plphys/kiae677

    Figure Lengend Snippet: ORA59 interacts with the ERFVIIs. A) Western blot analysis of RAP2.3 3xHA abundance in adult Arabidopsis plants leaves 48 h post infection with Botrytis followed by the quantification of the signal carried out using ImageLab TM software (BioRad). 40 μg of protein was loaded for each sample. Four biological replicates are shown. B) Expression analysis of ORA59 in Arabidopsis rosettes 48 h post infection. Data ( n = 6) are relative to the mock group set to 1. Statistically significant differences are indicated by the asterisks (Student t -test, unpaired comparison; * = P < 0.05; ** = P < 0.01; *** = P < 0.001; **** = P < 0.0001). C) Yeast two-hybrid assay between the 5 ERFVIIs and ORA59 (N-terminal 60 amino acids truncated, ORA59Δ1–60). D) As in C) , followed by a β-galactosidase assay (CPRG) test ( n = 4). E) Split-LUC assay of ORA59 fused to the N-terminal part of the firefly LUC and the ERFVIIs members (and negative control, HOL2) fused to the C-terminal part of the firefly LUC each ( n = 6). For D) and E) , statistically significant differences are indicated by asterisks after a one-way ANOVA followed by Dunnett's multiple comparisons test (* = P < 0.05; ** = P < 0.01; *** = P < 0.001; **** = P < 0.0001). Lines within the boxes indicate the median, while the bottom and top of each box denote the first and third quartile, respectively, the dots represent the single data points and whiskers denote the min/max values.

    Article Snippet: Affymetrix GeneChip Arabidopsis ATH1 Genome Arrays were used to process RNA, as previously described ( ).

    Techniques: Western Blot, Infection, Software, Expressing, Comparison, Y2H Assay, Negative Control

    ORA59 is a repressor of HRGs expression. A) Expression analysis of Group B and A core anaerobic genes in Col-0 and ora59 RNAi adult Arabidopsis rosette 48 h after inoculation with Botrytis. Data ( n = 6) are relative to the Col-0 mock set to 1. B) Expression of Group B and A core anaerobic genes in Col-0 protoplasts transformed with 35S:ORA59 and treated with gaseous hypoxia for 2 h. Data ( n = 4) are relative to the Col-0 untransformed control (air) set to 1. For A) and B) , different letters indicate significant difference ( P < 0.05) after two-ways ANOVA followed by Tukey's post-hoc test. C) Expression of ADH in erfVII protoplasts treated with gaseous hypoxia for 2 h. Protoplasts were transformed with each of the ERFVIIs singularly, under the control of the cauliflower mosaic virus 35S promoter, and with or without ORA59 driven by the same promoter. Statistically significant differences are indicated by asterisks after multiple unpaired t -test (* = P < 0.05; ** = P < 0.01; *** = P < 0.001; **** = P < 0.0001). Data ( n = 4) are relative to the erfVII untransformed group, set to 1. D) Inhibition of ERFVII-dependent expression of ADH by ORA59 in erfVII protoplasts. Data are the same as in and are mean ( n = 4) ±SD. Statistically significant differences are indicated by asterisks after a one-way ANOVA followed by Dunnett's multiple comparisons test (* = P < 0.05; ** = P < 0.01; *** = P < 0.001; **** = P < 0.0001). In box-plots, lines within the boxes indicate the median, while the bottom and top of each box denote the first and third quartile, respectively, the dots represent the single data points and whiskers denote the min/max values.

    Journal: Plant Physiology

    Article Title: The transcription factor ORA59 represses hypoxia responses during Botrytis cinerea infection and reoxygenation

    doi: 10.1093/plphys/kiae677

    Figure Lengend Snippet: ORA59 is a repressor of HRGs expression. A) Expression analysis of Group B and A core anaerobic genes in Col-0 and ora59 RNAi adult Arabidopsis rosette 48 h after inoculation with Botrytis. Data ( n = 6) are relative to the Col-0 mock set to 1. B) Expression of Group B and A core anaerobic genes in Col-0 protoplasts transformed with 35S:ORA59 and treated with gaseous hypoxia for 2 h. Data ( n = 4) are relative to the Col-0 untransformed control (air) set to 1. For A) and B) , different letters indicate significant difference ( P < 0.05) after two-ways ANOVA followed by Tukey's post-hoc test. C) Expression of ADH in erfVII protoplasts treated with gaseous hypoxia for 2 h. Protoplasts were transformed with each of the ERFVIIs singularly, under the control of the cauliflower mosaic virus 35S promoter, and with or without ORA59 driven by the same promoter. Statistically significant differences are indicated by asterisks after multiple unpaired t -test (* = P < 0.05; ** = P < 0.01; *** = P < 0.001; **** = P < 0.0001). Data ( n = 4) are relative to the erfVII untransformed group, set to 1. D) Inhibition of ERFVII-dependent expression of ADH by ORA59 in erfVII protoplasts. Data are the same as in and are mean ( n = 4) ±SD. Statistically significant differences are indicated by asterisks after a one-way ANOVA followed by Dunnett's multiple comparisons test (* = P < 0.05; ** = P < 0.01; *** = P < 0.001; **** = P < 0.0001). In box-plots, lines within the boxes indicate the median, while the bottom and top of each box denote the first and third quartile, respectively, the dots represent the single data points and whiskers denote the min/max values.

    Article Snippet: Affymetrix GeneChip Arabidopsis ATH1 Genome Arrays were used to process RNA, as previously described ( ).

    Techniques: Expressing, Transformation Assay, Control, Virus, Inhibition

    Production of ethanol is detrimental or Arabidopsis tolerance to Botrytis. A) Tolerance after Botrytis infection in Col-0, adh , pdc1pdc2 double mutants. The images show the lesion area 48 h after the inoculation. The scale bar is 1 cm. B) Percentage of lesion area normalized on the leaf size presented in a boxplot. Each dot represents the average value of a plant ( n = 12) (5 different infected leaves on each plant). C) Expression analysis in adult Arabidopsis rosette of B. cinerea actin A ( BcActinA ) as marker for the presence of the fungi. Data ( n = 6) are relative to Col-0 infected group set to 100 for BcActinA . For B) and C) , statistically significant differences are indicated by asterisks after a one-way ANOVA followed by Dunnett's multiple comparisons test (* = P < 0.05; ** = P < 0.01; *** = P < 0.001; **** = P < 0.0001). D) Transgenic line expressing GUS under the control of the ADH promoter ( pADH:GUS ). GUS staining was performed 48 h after the inoculation with Botrytis. The scale bar is 0.5 cm. E) Tolerance after Botrytis infection in Col-0 plants. Spores used in the infection were resuspended in either PDB (Control) or PDB added with 100 m M Ethanol. The images show the lesion area 48 h after the inoculation. The scale bar is 1 cm. F) Percentage of lesion area normalized on the leaf size presented in a boxplot. Each dot represents the average value of a plant ( n ≥ 9) (5 different infected leaves on each plant). G) Expression analysis in adult Arabidopsis rosette of B. cinerea actin A ( BcActinA ) as marker of the fungi presence. Data ( n = 9) are relative to Col-0 infected group set to 100 for BcActinA . For F) and G) , statistically significant differences are indicated by the asterisks (Student t -test, unpaired comparison; * = P < 0.05; ** = P < 0.01; *** = P < 0.001; **** = P < 0.0001). Lines within the boxes indicate the median, while the bottom and top of each box denote the first and third quartile, respectively, the dots represent the single data points and whiskers denote the min/max values.

    Journal: Plant Physiology

    Article Title: The transcription factor ORA59 represses hypoxia responses during Botrytis cinerea infection and reoxygenation

    doi: 10.1093/plphys/kiae677

    Figure Lengend Snippet: Production of ethanol is detrimental or Arabidopsis tolerance to Botrytis. A) Tolerance after Botrytis infection in Col-0, adh , pdc1pdc2 double mutants. The images show the lesion area 48 h after the inoculation. The scale bar is 1 cm. B) Percentage of lesion area normalized on the leaf size presented in a boxplot. Each dot represents the average value of a plant ( n = 12) (5 different infected leaves on each plant). C) Expression analysis in adult Arabidopsis rosette of B. cinerea actin A ( BcActinA ) as marker for the presence of the fungi. Data ( n = 6) are relative to Col-0 infected group set to 100 for BcActinA . For B) and C) , statistically significant differences are indicated by asterisks after a one-way ANOVA followed by Dunnett's multiple comparisons test (* = P < 0.05; ** = P < 0.01; *** = P < 0.001; **** = P < 0.0001). D) Transgenic line expressing GUS under the control of the ADH promoter ( pADH:GUS ). GUS staining was performed 48 h after the inoculation with Botrytis. The scale bar is 0.5 cm. E) Tolerance after Botrytis infection in Col-0 plants. Spores used in the infection were resuspended in either PDB (Control) or PDB added with 100 m M Ethanol. The images show the lesion area 48 h after the inoculation. The scale bar is 1 cm. F) Percentage of lesion area normalized on the leaf size presented in a boxplot. Each dot represents the average value of a plant ( n ≥ 9) (5 different infected leaves on each plant). G) Expression analysis in adult Arabidopsis rosette of B. cinerea actin A ( BcActinA ) as marker of the fungi presence. Data ( n = 9) are relative to Col-0 infected group set to 100 for BcActinA . For F) and G) , statistically significant differences are indicated by the asterisks (Student t -test, unpaired comparison; * = P < 0.05; ** = P < 0.01; *** = P < 0.001; **** = P < 0.0001). Lines within the boxes indicate the median, while the bottom and top of each box denote the first and third quartile, respectively, the dots represent the single data points and whiskers denote the min/max values.

    Article Snippet: Affymetrix GeneChip Arabidopsis ATH1 Genome Arrays were used to process RNA, as previously described ( ).

    Techniques: Infection, Expressing, Marker, Transgenic Assay, Control, Staining, Comparison

    The interplay between the ERFVIIs and ORA59 influences the reoxygenation phase after hypoxia. A) Expression analysis of ORA59 in Arabidopsis 5-d old seedlings during recovery in air for 5′ (R5′), 15′ (R15′), 30′ (R30′), and 1 h (R1) after 4 h of gaseous hypoxia (H4). Data ( n = 5) are relative to aerobic (T0) seedlings set to 1. B) LUC activity in 35S:RAP2.12 1–28 -LUC 7-d old seedling during post-hypoxia recovery in air for 1 h (R1), 2 h (R2), and 3 h (R3). For A) and B) , statistically significant differences ( n = 96) are indicated by the asterisks after a one-way ANOVA followed by Dunnett's multiple comparisons test (* = P < 0.05; ** = P < 0.01; *** = P < 0.001; **** = P < 0.0001). C) Expression analysis of Group B and A core anaerobic genes during recovery after 4 h of gaseous hypoxia in Col-0 and ora59 RNAi 5-d old seedlings. Curved lines represent polynomial fits passing through the mean ( n = 6) of each group of observations, providing a visual representation of the trend within each genotype. Statistically significant differences are assessed using two-ways ANOVA followed by Sidak's multiple comparisons test (* = P < 0.05; ** = P < 0.01; *** = P < 0.001; **** = P < 0.0001). Lines within the boxes indicate the median, while the bottom and top of each box denote the first and third quartile, respectively, the dots represent the single data points and whiskers denote the min/max values.

    Journal: Plant Physiology

    Article Title: The transcription factor ORA59 represses hypoxia responses during Botrytis cinerea infection and reoxygenation

    doi: 10.1093/plphys/kiae677

    Figure Lengend Snippet: The interplay between the ERFVIIs and ORA59 influences the reoxygenation phase after hypoxia. A) Expression analysis of ORA59 in Arabidopsis 5-d old seedlings during recovery in air for 5′ (R5′), 15′ (R15′), 30′ (R30′), and 1 h (R1) after 4 h of gaseous hypoxia (H4). Data ( n = 5) are relative to aerobic (T0) seedlings set to 1. B) LUC activity in 35S:RAP2.12 1–28 -LUC 7-d old seedling during post-hypoxia recovery in air for 1 h (R1), 2 h (R2), and 3 h (R3). For A) and B) , statistically significant differences ( n = 96) are indicated by the asterisks after a one-way ANOVA followed by Dunnett's multiple comparisons test (* = P < 0.05; ** = P < 0.01; *** = P < 0.001; **** = P < 0.0001). C) Expression analysis of Group B and A core anaerobic genes during recovery after 4 h of gaseous hypoxia in Col-0 and ora59 RNAi 5-d old seedlings. Curved lines represent polynomial fits passing through the mean ( n = 6) of each group of observations, providing a visual representation of the trend within each genotype. Statistically significant differences are assessed using two-ways ANOVA followed by Sidak's multiple comparisons test (* = P < 0.05; ** = P < 0.01; *** = P < 0.001; **** = P < 0.0001). Lines within the boxes indicate the median, while the bottom and top of each box denote the first and third quartile, respectively, the dots represent the single data points and whiskers denote the min/max values.

    Article Snippet: Affymetrix GeneChip Arabidopsis ATH1 Genome Arrays were used to process RNA, as previously described ( ).

    Techniques: Expressing, Activity Assay

    Expression profiles of genes encoding enzymes related to starch metabolism during the SD diurnal cycle. Genes are grouped by their biochemical activity: ( A ) starch synthases (SSs), ( B ) starch branching enzymes (BEs), ( C ) starch debranching enzymes (DBEs), ( D ) starch disproportionating enzymes (DPEs), ( E ) β-amylases (BAMs), ( F ) α-amylases (AAMs), ( G ) starch phosphorylases (PHSs), and ( H ) other enzymes related to starch metabolism. Leaves from WT plants cultivated under short day (SD) conditions, consisting of 8 h of light followed by 16 h of dark, were collected at various times throughout the 24-h cycle. RNA from these samples was then examined using the Affymetrix Arabidopsis ATH1 chip. The average normalized intensity for each microarray chip is 1. RNA was analyzed using GeneChip. The analysis was repeated in two independent experiments.

    Journal: International Journal of Molecular Sciences

    Article Title: Modulation of the Arabidopsis Starch Metabolic Network by the Cytosolic Acetyl-CoA Pathway in the Context of the Diurnal Illumination Cycle

    doi: 10.3390/ijms251910850

    Figure Lengend Snippet: Expression profiles of genes encoding enzymes related to starch metabolism during the SD diurnal cycle. Genes are grouped by their biochemical activity: ( A ) starch synthases (SSs), ( B ) starch branching enzymes (BEs), ( C ) starch debranching enzymes (DBEs), ( D ) starch disproportionating enzymes (DPEs), ( E ) β-amylases (BAMs), ( F ) α-amylases (AAMs), ( G ) starch phosphorylases (PHSs), and ( H ) other enzymes related to starch metabolism. Leaves from WT plants cultivated under short day (SD) conditions, consisting of 8 h of light followed by 16 h of dark, were collected at various times throughout the 24-h cycle. RNA from these samples was then examined using the Affymetrix Arabidopsis ATH1 chip. The average normalized intensity for each microarray chip is 1. RNA was analyzed using GeneChip. The analysis was repeated in two independent experiments.

    Article Snippet: Synthesis of labeled cRNAs, hybridization with Affymetrix Arabidopsis ATH1 arrays (22,810 Arabidopsis probe sets, Affymetrix, Santa Clara, CA, USA), and scanning of the probe arrays were performed at the Iowa State University Gene Chip Facility (Ames, IA, USA).

    Techniques: Expressing, Starch, Activity Assay, Microarray

    Expression correlations among genes within the starch metabolic network. Expression correlations among starch metabolic genes were evaluated by 963 Affymetrix ATH1 microarray chips representing 70 different Arabidopsis experiments and analyzed by MOG . Pearson correlation values are color-coded. Vertical bars (labeled #1, group 1; #2, group 2; and #3, group 3) denote the three groups of genes with the highest correlations. Group 1 represents genes involved in starch degradation, Group 2 includes genes primarily associated with starch synthesis, and Group 3 comprises genes involved in both starch synthesis and carbohydrate metabolism. Details for each group are described in the text.

    Journal: International Journal of Molecular Sciences

    Article Title: Modulation of the Arabidopsis Starch Metabolic Network by the Cytosolic Acetyl-CoA Pathway in the Context of the Diurnal Illumination Cycle

    doi: 10.3390/ijms251910850

    Figure Lengend Snippet: Expression correlations among genes within the starch metabolic network. Expression correlations among starch metabolic genes were evaluated by 963 Affymetrix ATH1 microarray chips representing 70 different Arabidopsis experiments and analyzed by MOG . Pearson correlation values are color-coded. Vertical bars (labeled #1, group 1; #2, group 2; and #3, group 3) denote the three groups of genes with the highest correlations. Group 1 represents genes involved in starch degradation, Group 2 includes genes primarily associated with starch synthesis, and Group 3 comprises genes involved in both starch synthesis and carbohydrate metabolism. Details for each group are described in the text.

    Article Snippet: Synthesis of labeled cRNAs, hybridization with Affymetrix Arabidopsis ATH1 arrays (22,810 Arabidopsis probe sets, Affymetrix, Santa Clara, CA, USA), and scanning of the probe arrays were performed at the Iowa State University Gene Chip Facility (Ames, IA, USA).

    Techniques: Expressing, Starch, Microarray, Labeling

    Gene expression correlated with the expression of the DPE2 gene. Gene-expression profiles of nine genes ( PTPKIS1 / SEX4 , GWD1 , ISA3 , GWD3 , ISA2 , PHS2 , PHS1 , AT1G06460, and AT2G31040) correlated to the expression profile of DPE2 (Pearson’s correlation coefficients of between 0.72 and 0.84). Gene expression was evaluated at the level of RNA accumulation from 963 Affymetrix ATH1 microarray chips representing 70 different Arabidopsis experiments and analyzed by MOG ; the average expression level for each chip is 6.6.

    Journal: International Journal of Molecular Sciences

    Article Title: Modulation of the Arabidopsis Starch Metabolic Network by the Cytosolic Acetyl-CoA Pathway in the Context of the Diurnal Illumination Cycle

    doi: 10.3390/ijms251910850

    Figure Lengend Snippet: Gene expression correlated with the expression of the DPE2 gene. Gene-expression profiles of nine genes ( PTPKIS1 / SEX4 , GWD1 , ISA3 , GWD3 , ISA2 , PHS2 , PHS1 , AT1G06460, and AT2G31040) correlated to the expression profile of DPE2 (Pearson’s correlation coefficients of between 0.72 and 0.84). Gene expression was evaluated at the level of RNA accumulation from 963 Affymetrix ATH1 microarray chips representing 70 different Arabidopsis experiments and analyzed by MOG ; the average expression level for each chip is 6.6.

    Article Snippet: Synthesis of labeled cRNAs, hybridization with Affymetrix Arabidopsis ATH1 arrays (22,810 Arabidopsis probe sets, Affymetrix, Santa Clara, CA, USA), and scanning of the probe arrays were performed at the Iowa State University Gene Chip Facility (Ames, IA, USA).

    Techniques: Expressing, Microarray